the method of procedure and the details of any mechanical process or surgical operation. See also under maneuver, method, operation, procedure, treatment, stain, test, etc.
Alexander technique, a bodywork technique that uses psychophysical reeducation to correct dysfunctional habits of posture and movement, based on the principle that human movement is most fluid when the head leads and the spine follows, in order to improve postural balance, coordination, and breathing function; relieve stress and chronic pain; and improve general well-being.
Amplatz technique, a femoral approach for coronary arteriography similar to the Judkins technique but using preformed Amplatz right or left coronary catheters.
aseptic technique, any procedure designed to keep a surgical field as nearly aseptic as possible, e.g., gloving of the surgeon and aides, draping of the patient, autoclaving of instruments, and proper disposal of waste. Called also sterile t.
Begg technique, an orthodontic technique employing a fixed multibanded appliance that incorporates a concept of differential light forces and uses a modified ribbon arch attachment and elastics (Begg appliance). Tipping the crowns of teeth to be moved, rather than moving them laterally, is used in the technique, thereby minimizing the use of orthodontic force.
Bowen technique, a bodywork technique in which soft tissue mobilization is performed by gentle pressure from the thumb and fingers; used primarily for musculoskeletal conditions and stress-related disorders and for symptomatic relief in chronic conditions.
Bricker technique, see under procedure.
Brown-Roberts-Wells technique, a stereotactic technique that uses a ring to hold the head in position, a ring for localization of a computed tomography image, and an arc guidance system.
clamp technique, see clamping.
Cohen technique, cross-trigonal technique, a type of ureteroneocystostomy in which the ureter is excised from its attachment to the bladder and reimplanted in a new submucosal tunnel slightly above the bladder trigone.
dilution-filtration technique, a blood culture technique in which any culture inhibitors present in the blood are diluted out and red blood cells are removed before the sample is filtered and cultured, thereby permitting the identification of organisms in about 24 hours.
DNA amplification technique, see nucleic acid amplification t..
dye dilution technique, see under method.
enzyme-multiplied immunoassay technique, see EMIT.
fluorescent antibody technique, an immunofluorescence technique in which antigen in tissue sections is located by homologous antibody labeled with fluorochrome (the single-layer technique) or by treating the antigen with unlabeled antibody followed by a second layer of labeled antiglobulin which is reactive with the unlabeled antibody (double-layer technique). Variations include direct, indirect, inhibition, and complement staining techniques.
gene amplification technique, a term sometimes used to denote a nucleic acid amplification technique, although the segment of DNA or RNA undergoing amplification does not necessarily correspond to a single, entire gene.
Gil-Vernet technique, a type of ureteroneocystostomy in which both ureters are excised from their normal attachments to the bladder and reattached medially near each other within the trigone.
Glenn-Anderson technique, a type of ureteroneocystostomy in which a damaged ureteral attachment site is repaired in the original location.
hanging drop technique, a method of microscopic examination of organisms suspended in a drop on a special concave microscope slide.
immunoperoxidase technique, a method of histologic staining in which a peroxidase-labeled antibody that binds to antigen is added to tissue, and the sites of its localization are revealed by addition of a chromogenic substrate system that produces a colored reaction product visible by light microscopy. Cf. peroxidase-antiperoxidase (PAP) t.
indicator dilution technique, see under method.
Irving technique, a method of tubal ligation in which the uterine tubes are ligated and severed and the proximal ends are sewn into the myometrium. Called also Irving operation.
isolation-perfusion technique, a technique for administering high doses of a chemotherapy agent to a region while protecting the patient from toxicity: the blood flow of the region is isolated, as by application of a tourniquet to an extremity, and the region is perfused by means of a pump-oxygenator; the drug is added to the perfusate, which may be heated by a heat exchanger to provide hyperthermia.
Jerne plaque technique, a hemolytic technique for detecting antibody-producing cells: a suspension of presensitized lymphocytes is mixed in an agar gel with erythrocytes; after a period of incubation, complement is added and a clear area of lysis of red cells can be seen around each of the antibody-producing cells.
Judkins technique, a femoral approach for coronary arteriography, in which a preformed Judkins right or left coronary catheter is inserted into the right or left coronary artery, respectively, via percutaneous cannulation of the femoral artery.
Kleinschmidt technique, rupture of the virion by osmotic shock so that viral DNA is exposed.
Laurell technique, crossed immunoelectrophoresis (Laurell first technique).rocket immunoelectrophoresis (Laurell second technique).
Leboyer technique, see under method.
LeDuc technique, a type of ureterointestinal anastomosis in which the ureter is brought through the bowel wall and the end is spatulated and sutured to a T-shaped incision in the bowel mucosa. Called also LeDuc implantation.
Leksell technique, a stereotactic technique that uses an arc guidance system and a cube-shaped frame that holds the head in position and is marked with X, Y, and Z coordinates for three-dimensional orientation.
Lich technique, Lich-Gregoir technique, a type of ureteroneocystostomy in which the ureter is excised from its attachment to the bladder and reattached to a submucosal tunnel created within the bladder.
membrane filter technique, a technique for detecting bacteria in a sample of water: the sample is passed through a membrane filter, leaving bacteria on the filter surface; that surface is then placed on culture medium, incubated, and analyzed for bacterial colonies.
Mohs technique, see under surgery.
needle-through-needle technique, a technique of anesthetic administration in which a narrow spinal needle is advanced through the lumen of a larger-gauge needle such as a Tuohy needle and past its tip to puncture the dura so that spinal and epidural anesthesia can be administered at the same time.
nucleic acid amplification technique, any of various in vitro methods by which a DNA or RNA sequence is amplified, making it more readily detectable for various procedures or tests. The original, and still most commonly used, is the polymerase chain reaction. See also under test.
Oakley-Fulthorpe technique, double diffusion in one dimension; see under diffusion.
Orr technique, see under treatment.
Ouchterlony technique, double diffusion in two dimensions; see under diffusion.
Oudin technique, a single diffusion (see under diffusion) technique in which agar containing antiserum is placed in a test tube and antigen is layered over it; precipitin lines form where the concentrations of each antigen and antibody are equivalent.
PAP technique, peroxidase-antiperoxidase t.
Paquin technique, a type of ureteroneocystostomy in which the ureter is excised from its attachment to the bladder and reattached in a more posteromedial position.
peroxidase-antiperoxidase technique, a technique for detecting antigen or antibody in tissue sections. The tissue section is incubated with rabbit antibody specific for the antigen to be detected, followed by an excess of antirabbit IgG. A complex of horseradish peroxidase and rabbit antiperoxidase is added; these are linked to the antigen-bound antibody by the antirabbit IgG. The PAP complexes are then stained by incubation with a chromogenic substrate to produce a colored reaction product. Called also PAP t.
Politano-Leadbetter technique, a type of ureteroneocystostomy in which the ureter is excised from its attachment to the bladder and reattached in a more medial and superior position.
Pomeroy technique, a method of tubal ligation in which a loop of fallopian tube is picked up and ligated at its base with an absorbable suture, about 5 cm from the uterine cornua, and the tied loop is then resected. Called also Pomeroy operation.
push-back technique, a surgical procedure designed to reposition the soft palate posteriorly and reestablish velopharyngeal competence. Called also push-back procedure.
Ravitch technique, a surgical technique for the repair of pectus excavatum, in which the deformed costal cartilages are resected with preservation of the perichondrium, from which new cartilage regenerates, and correction of the sternal deformity.
Rebuck skin window technique, a technique used to study the inflammatory process; an area of skin is abraded until capillary bleeding occurs and a coverslip or chamber containing balanced salt solution is applied. This permits direct observation of inflammatory cells migrating into the site; polymorphonuclear leukocytes predominate at about 10 hours; macrophages predominate at about 4 days.
Riechert-Mundinger technique, a stereotactic technique that uses a semicircular arc guidance system and a ring to hold the head in position.
RNA amplification technique, see nucleic acid amplification t..
sandwich technique, detection of a specific molecule, as a protein or nucleic acid, by sandwiching it between two layers of a different molecule or material, the second of which is radiolabeled or otherwise tagged for detection. The term is sometimes used specifically to denote detection of an antigen using two layers of antibody.any technique that places something of interest between two layers of something else more easily detected or performed, or otherwise useful.
Schuster technique, a method for repair of an omphalocele consisting of covering the sac with prosthetic sheeting followed by progressive reduction of the contents into the abdominal cavity.
scintillation counting technique, a method of determining the amount of radioactivity by use of a scintillation counter (q.v.).
Seldinger technique, a method for introducing a catheter into a hollow lumen structure or body cavity; a narrow needle is used to enter the structure, a guidewire is passed through the needle, the needle is removed, and the catheter is advanced over the wire. Used in angiography, cardiac catheterization, and cannulation of the central venous system.
shoelace technique, a technique for delayed primary closure of fasciotomy: skin staples placed 2 cm apart serve as eyes for a loop of suture material that serves as the lace; daily tightening produces gradual reapproximation of the skin edges.
Sones technique, a brachial approach for coronary arteriography, in which the catheter is inserted via a brachial arteriotomy; usually a single Sones catheter can be used in either coronary artery or for entry into the left ventricle.
sperm swim-up technique, any of several methods of checking sperm for motility; a semen sample is centrifuged to form pellets, which are then covered with culture medium. The spermatozoa with greatest motility will swim up into the culture medium and be more suitable for use in in vitro fertilization.
squash technique, any of a variety of techniques for preparation of a tissue or other histologic sample for microscopic examination, including treatment and staining of the sample, placement on a slide, and finally “squashing” down of the coverslip to flatten and spread the material.
squeeze technique, a technique used for treatment of premature ejaculation; the patient is repeatedly aroused almost to the point of ejaculatory inevitability and then the thumb and first two fingers are used to forcibly squeeze the head of the penis, preventing ejaculation.
stereotactic technique, any of the techniques used to perform stereotactic surgery, such as the Brown-Roberts-Wells technique, Leksell technique, Riechert-Mundinger technique, or Todd-Wells technique. Called also stereotactic system.
sterile technique, aseptic t.
thermal dilution technique, thermodilution technique, thermodilution.
Todd-Wells technique, a stereotactic technique that uses an arc-quadrant, a ring around the head to steady the head and provide one set of reference angles, and a ring around the neck to provide a second set of reference angles.
transfixion technique, a percutaneous approach for arterial or venous access; using a needle within a catheter, the posterior wall of the artery is punctured, the needle removed, and the catheter slowly withdrawn until blood flows freely.
Trueta technique, see under treatment.
Uchida technique, a method of tubal ligation with injection of saline solution beneath the tubal mucosa to separate it from the underlying tube; a portion of mucosa is removed and the mucosa-free tube then retracts to form a stump that is closed with sutures.
ultrasound dilution technique, a technique for measuring blood flow and access recirculation in hemodialysis patients: ultrasound sensors are attached to the venous and arterial catheters in their normal positions and blood flow is checked; then they are reversed, the ultrafiltration is turned off, and at a known pumped blood flow rate a bolus of saline is released into the venous catheter to dilute the blood; the velocity of the dilution as it passes through the access apparatus is measured by ultrasonography.
Western blot technique, see Western blot, under blot.
Pomeroy technique. (A), Tube is grasped with forceps; (B), a loop is ligated; (C), the loop is excised; (D), several months later, the fibrosed ends of the tube separate.